- Title
- Relationship of transcriptional markers to Leydig cell number in the mouse testis
- Creator
- Rebourcet, Diane; Monteiro, Ana; Cruickshanks, Lyndsey; Jeffery, Nathan; Smith, Sarah; Milne, Laura; O'Shaughnessy, Peter J.; Smith, Lee B.
- Relation
- PL o S One Vol. 14, Issue 7, no. e0219524
- Publisher Link
- http://dx.doi.org/10.1371/journal.pone.0219524
- Publisher
- Public Library of Science (PLoS)
- Resource Type
- journal article
- Date
- 2019
- Description
- Objectives: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size. Results: Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.
- Subject
- transcriptional markers; Leydig cells; testis; stereological cell counting
- Identifier
- http://hdl.handle.net/1959.13/1444081
- Identifier
- uon:42192
- Identifier
- ISSN:1932-6203
- Language
- eng
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